Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Cayman公司成立于1980年，在过去20多年的发展过程中，始终保持着为全球科研工作者提供高质、优价研究产品的宗旨。该公司目前可以提供 2,138 多种生化和免疫试剂，涉及肿瘤、氧化氮、神经学、凋亡、氧化性损伤、内分泌学等研究领域。非常有特色的检测试剂盒包括类花生酸类物质、游离的生物标志、环核苷、激素及氧化氮等。此外，还提供多种高质量试剂，如类花生酸类物质、氧化氮试剂及许多相关脂质、脂肪酸、酶和抗体等。Cayman非常愿意接受各地客户的要求，合成复杂和不稳定分子。

Cayman Chemical Company is helping make research possible by supplying scientists worldwide with biochemical tools in research disciplines such as cancer, nitric oxide, neuroscience, apoptosis, oxidative injury, endocrinology, and much more. We specialize in assay kits for the measurement of eicosanoids, free radical biomarkers, cyclic nucleotides, cytokines, hormones, and nitric oxide. In addition, Cayman offers a broad range of quality biochemicals including eicosanoids, nitric oxide reagents, and a variety of related lipids, fatty acids, enzymes, and antibodies.

Custom biochemical and organic synthesis is also available upon request, and we grant additional discounts for bulk orders.

3,370 different biochemicals (March, 2009) are manufactured and analyzed on site at our 66,000 square foot facility in Ann Arbor, Michigan. We have recently expanded the EIA, organic, and biochemistry departments, and have established a new laboratory dedicated to GMP Production.

Cayman is frequently the only manufacturer in the world willing to undertake the synthesis of the most complex and unstable molecules.

By coupling that determination with an affordable pricing policy, we support our commitment to human healthcare research.
Cayman Chemical Company was incorporated June 6, 1980 in Denver, Colorado. The goal of the new business was to demonstrate the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Careful environmental studies and negotiations with the Grand Cayman government culminated in August of 1981, when an eight pound sample of the gorgonian Plexaura homomalla was collected near Fisherman's Cay in the North Sound of Grand Cayman Island. The coral was frozen and transported to a small lab in Denver where 30 grams of relatively pure Prostaglandin A2 was extracted. Inspired by this success and the vision of producing affordable, high quality prostaglandins to the research community, the new laboratory printed and mailed a flier offering five prostaglandin standards. In November of 1981, Cayman Chemical closed its first sale.

Growth
After several years of growth, the fledgling company moved to Ann Arbor, Michigan, and established its first complete synthetic chemistry laboratory. From there, operations expanded along with the rapidly growing field of eicosanoid research. Product growth was accelerated by the acquisition of AIA Reagents and the addition of their eicosanoid Enzyme Immunoassy kits to Cayman's product line. Another significant growth spurt came in 1998 with the purchase of our 40,000 square foot research laboratory. A short five years later, we added to capacity again by acquiring a neighboring parcel, bringing the Cayman Campus to 14 acres. Today 3,370 different biochemicals (March, 2009) are manufactured and analyzed on site at our 66,000 square foot facility. We are actively recruiting organic synthetic chemists, and are planning a new, dedicated facility to triple our GMP production capacity.

Support for the Research Community
Cayman has made an ongoing committment to assist young research scientists in new ways that complement the molecular tools we already provide. We now offer travel funds and support to graduate and post-doctoral students who may fill out a support request. While we can't guarantee that we can fund all of the requests, we have already donated many thousands of dollars directly to support the attendance of young scientists at important meetings.

Today
Though Cayman Chemical has changed dramatically over the years, we remain firmly committed to our original goal of providing high quality, affordable products to researchers worldwide. In addition to the Cayman name, other elements of the company origin have been incorporated into our daily business operations to remind us of our corporate history and the founding vision. Although most of our biochemicals are now manufactured by total synthesis, we continue to express appreciation for marine ecosystems by featuring them in our promotional material whenever possible. Their presence serves as a reminder of the significant contributions marine organisms have made to science and the importance of preserving their habitats.